Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells

Figure 6

Effects of test compounds and kojic acid on cellular melanin content in a-MSH-stimulated B16 melanoma cells compared with kojic acid. The cells were incubated with 100 μM alone or together with increasing doses of tested compounds or kojic acid for 72 h following which total cellular melanin activity was measured. Baseline melanin content in control wells not exposed to a-MSH and any test compounds or kojic acid was set at 100%. Data from experimental wells were expressed as a percentage of control. Each column represents the mean ± SD of three independent experiments. versus control group (without a-MSH). , , and versus a-MSH-treated group.