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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 281508, 13 pages
Research Article

Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract

Department of Biological and Environmental Science and Technology (Di.S.Te.B.A.), University of Salento, Prov.le Lecce-Monteroni, 73100 Lecce, Italy

Received 22 August 2013; Accepted 31 December 2013; Published 25 March 2014

Academic Editor: Mohamed Eddouks

Copyright © 2014 Elisa Panzarini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of the C. papaya seeds water extract against hydrogen peroxide (H2O2) oxidative stress in human skin Detroit 550 fibroblasts. C. papaya seeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2 oxidative stress. Data show that (i) the maximum protective effect is achieved by the simultaneous administration of the extract with 1 mM H2O2; (ii) the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential loss; (iii) the extract is more efficient than vitamin C to hamper the oxidative damage; (iv) the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion, C. papaya seeds water extract is potentially useful for protection against oxidative stress.