Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract
Figure 3
Antioxidant activity of C. papaya seeds water extract on Detroit 550 fibroblasts. (a-b) Viability was assessed by MTT assay. The values are reported as percentage of the control untreated fibroblasts considered as 100%. (a) Detroit 550 fibroblasts were treated with 1 or 2 mg/mL C. papaya (Cp) seeds water extract simultaneously with 1 mM H2O2 for 1 h or during the recovery. Single star indicates values significantly different from untreated control fibroblasts (). (b) Detroit 550 fibroblasts were treated with different concentrations of vitamin C (vit C) simultaneously with 1 mM H2O2 for 1 h or during the recovery. Single star indicates that the value is significantly different from all others (). Two stars indicate values significantly different from those marked with one star and from the simultaneous treatment value (). (c) Catalase enzyme activity (U/mL) measured in Detroit 550 fibroblasts treated with 1 mg/mL of Cp seeds water extract added simultaneously (s) with 1 mM H2O2 for 1 h or during recovery (r). Single star shows values significantly different from all others (). (d) Percentages of normal and apoptotic Detroit 550 fibroblasts after treatment with 1 mM H2O2 administrated either alone or in combination with 1 or 2 mg/mL of Cp extract or with Cp extract added during 1 h recovery. At least 500 fibroblasts for each treatment were scored on H-E stained slides. Single star shows a value significantly different from all others and from the corresponding apoptotic untreated control fibroblasts (). Two stars indicate values significantly different from the untreated control fibroblasts and from the corresponding apoptotic ones (). Each value represents the mean ± SE of six independent experiments, each done in duplicate.
