Differential Effects of Naja naja atra Venom on Immune Activity
Figure 3
NNAV inhibited T-cell proliferation. After 21-day administration of NNAV or water, splenocytes were isolated from NNAV-treated or control ICR mice. (a) Splenocytes were stimulated with ConA (5 μg/mL) for 48 h, and the proliferation rate of T cells was determined using CCK-8. Data present mean values ± SD for 4 mice per group; triplicates were used for each mouse. (versus control). (b) Splenocytes were stained with CFSE and stimulated with ConA (5 μg/mL) for 72 h. The cell signals were obtained with or without gating on CD4+ or CD8+ T subsets with flow cytometer. The cell division was represented by the histograms. This is representative of three experiments. (c) Splenocytes were stimulated with ConA (5 μg/mL) for 48 h. Then cells were fixed with 70% ethanol and stained with PI for the cell cycle phase distribution analysis with flow cytometer. Data represent mean values ± SD for 6 mice per group. (versus control).
