Research Article

Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice

Figure 3

EORP-induced reduction in the upregulation of IL-1β expression in LPS-treated HASMCs is mediated by inhibition of both NF-κB p65 phosphorylation and nuclear translocation. (a, b) Western blotting and immunofluorescent staining for NF-κB p65. HASMCs were left untreated or incubated for 24 h with or without 10 μg/mL EORP and then with or without 10 μg/mL of LPS for 90 min in the continued presence of the EORP. (a) The phosphorylation of NF-κB p65 expression is examined by Western blotting. (b) NF-κB p65 expression is indicated by green fluorescence (FITC) and nuclei by blue fluorescence (DAPI). A representative result from three separate experiments is shown. Bar = 100 μm. (c) Cells were incubated for 1 h with 30 μM parthenolide (NF-κB inhibitor) and then coincubated for 24 h with 10 μg/mL of LPS, and then cell lysates were prepared and assayed for IL-1β on Western blots. The data are the mean ± SEM ( ). * as compared to the untreated (control) cells; as compared to the LPS-treated cells.
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