Evidence-Based Complementary and Alternative Medicine

Review Article

A Review of Botanical Characteristics, Traditional Usage, Chemical Components, Pharmacological Activities, and Safety of Pereskia bleo (Kunth) DC

Table 6

IC₅₀ values (g/mL) of P. bleo leaf extracts and fractions on different cell lines.


Cell line	Extracts and fractions (IC₅₀: g/mL)					Positive control (IC₅₀: g/mL)	Negative control	References
Cell line	Methanol	Water	Hexane	Dichloromethane	Ethylacetate	Positive control (IC₅₀: g/mL)	Negative control	References

4T1	>50	>50	NA	NA	NA	Cisplatin (NA)	NA	[32]
CasKi	40.5	—	89.5	NA	58	Doxorubicin (6 × 10⁻³)	NA	[36]
CEM-ss	—	NA	—	—	—	NA	VC	[8]
HT29 and HCT116	>30	NA	>30	>30	>30	NA	VC	[8]
HT29 and HCT116	41.6	—	67.5	NA	22	Doxorubicin (3.6 × 10⁻¹)	NA	[36]
KB	6.5	—	28	NA	4.5	Doxorubicin (1.2 × 10⁻²)	NA	[36]
MCF-7	>30	NA	>30	>30	>30	NA	VC	[8]
MCF-7	39	—	25	NA	28	Doxorubicin (7.5 × 10⁻²)	NA	[36]
MRC-5	—	—	—	NA	—	Doxorubicin (5.5 × 10⁻¹)	NA	[36]
NIH/3T3	≥200	≥200	NA	NA	NA	Cisplatin	NA	[32]
Saos-2	—	NA	NA	NA	NA	Cisplatin	NA	[39]
T-47D	2	NA	NA	NA	NA	DNase I	VC	[40]
V79	—	NA	NA	NA	NA	Nitracrine	VC	[22]

IC₅₀: 50% of maximum cell inhibition. IC₅₀ < 20 g/mL is considered active, 100 > IC₅₀ > 20 g/mL is relatively active, and IC₅₀ > 100 is not active [8].
(—): no activity.
4T1: mouse mammary cancer cell line; CasKi: human cervical carcinoma cell line; CEM-ss: human T-4 lymphoblastoid cell line; HT29 and HCT116: human colon carcinoma cell line; KB: human nasopharyngeal epidermoid carcinoma cell line; MCF-7: hormone dependent breast carcinoma cell line; MRC-5: normal human fibroblast cell lines; NIH/3T3: normal mouse fibroblast cell line; Saos-2: human osteosarcoma cell line; T-47D: human breast carcinoma cell line; V79: Chinese hamster lung fibroblasts.
NA: not available.
VC: vehicle control.