Inhibitory Effect of a Hot-Water Extract of Leaves of Japanese Big-Leaf Magnolia (Magnolia obovata) on Rotavirus-Induced Diarrhea in Mouse Pups

Figure 7

Anti-RV agents in 1-BuOH-soluble fraction of MLE. (a) HPLC profiles of 1-BuOH-soluble fraction of MLE, quercitrin, and rutin and quercetin. The x-axis represents time and the y-axis represents voltage. (b) Structures of quercitrin and rutin. (c) Effect of quercitrin and rutin on the amplification of viral protein mRNA in SA11-infected MA104 cells. MA104 cells were treated with quercitrin and rutin 1 h after the exposure to SA11 strain. The SA11-infected cells were cultured for another 8 h and subjected to quantitative RT-PCR assay to evaluate mRNA expression of VP6 and NSP4. The results of untreated (control) cells, quercitrin-treated cells, and rutin-treated cells were shown by dotted bar, shaded bar, and solid black bar, respectively. Data are presented as relative mean value ± SD against untreated control after 8 h (). and versus untreated counterpart.

(a)

(b)

(c)