Antioxidative Effects of Germinated Brown Rice-Derived Extracts on H₂O₂-Induced Oxidative Stress in HepG2 Cells

Table 1

Gene name and sequences of primers used in the multiplex panel.

Gene name	Forward primer sequence	Reverse primer sequence
SOD 1	AGGTGACACTATAGAATATCATCAATTTCGAGCAGAAGG	GTACGACTCACTATAGGGATGCTTTTTCATGGACCACC
SOD 2	AGGTGACACTATAGAATACATCAAACGTGACTTTGGTTC	GTACGACTCACTATAGGGACTCAGCATAACGATCGTGGTT
Catalase	AGGTGACACTATAGAATAGAAGTGCGGAGATTCAACACT	GTACGACTCACTATAGGGAACACGGATGAACGCTAAGCT
AKT	AGGTGACACTATAGAATAGAGGAGATGGACTTCCGGTC	GTACGACTCACTATAGGGAAGGATCTTCATGGCGTAGTAGC
P53	AGGTGACACTATAGAATAGGGGAGCAGGGCTCA	GTACGACTCACTATAGGGAAAAATGGCAGGGGAGGG
P38 MAPK	AGGTGACACTATAGAATATTCAGTCTTTGACTCAGATGCC	GTACGACTCACTATAGGGAGTCAGGCTTTTCCACTCATCT
JNK	AGGTGACACTATAGAATACAGAAGCTCCACCACCAAAGAT	GTACGACTCACTATAGGGAGCCATTGATCACTGCTGCAC
BAX	AGGTGACACTATAGAATACCCTTTTGCTTCAGGGTTTC	GTACGACTCACTATAGGGACAAAGTAGAAAAGGGCGACAA
CDK4	AGGTGACACTATAGAATAGTCAAGCTGGCTGACTTTGG	GTACGACTCACTATAGGGATCGACGAAACATCTCTGCAA
Peptidylprolyl isomerase A^*#	AGGTGACACTATAGAATACACACGGCTCACATTGCAT	GTACGACTCACTATAGGGACACGAACAGCAAAGCGA
Beta actin^*	AGGTGACACTATAGAATAGATCATTGCTCCTCCTGAGC	GTACGACTCACTATAGGGAAAAGCCATGCCAATCTCATC
Glyceraldehyde-3-phosphate dehydrogenase^*	AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA	GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG

Housekeeping genes. ^#Used for normalization. Reverse transcription (RT) and PCR were done according to manufacturer’s instructions; RT reaction was at 48°C for 1 min; 37°C for 5 min; 42°C for 60 min; 95°C for 5 min; then hold at 4°C, while PCR was as follows: initial denaturation at 95°C for 10 min, followed by two-step cycles of 94°C for 30 sec and 55°C for 30 sec, ending in a single extension cycle of 68°C for 1 min. AKT: v-akt murine thymoma viral oncogene homolog 1; BAX: Bcl-2-associated X; CDK4: cyclin-dependent kinase 4; JNK: c-Jun N-terminal kinases; p38 MAPK: p38 mitogen-activated protein kinase; p53: tumor suppressor p53; SOD: superoxide dismutase.