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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 379890, 8 pages
http://dx.doi.org/10.1155/2014/379890
Research Article

Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage

1Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, IPN, Unidad Profesional A. López Mateos, Avenida Wilfredo Massieu s/n, Zacatenco, Colonia, Lindavista, CP 07738, México, DF, Mexico
2Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados, IPN, Avenida Instituto Politécnico 2508, Colonia San Pedro Zacatenco, Del. Gustavo A. Madero, CP 06360, México, DF, Mexico
3Laboratorio de Medicina de la Conservación, Escuela Superior de Medicina, IPN, Plan de San Luis y Díaz Mirón s/n, Casco de Santo Tomás, Del. Miguel Hidalgo, CP 11340, México, DF, Mexico
4Laboratorio de Química Ambiental. Escuela Nacional de Ciencias Biológicas, IPN, Unidad Profesional A. López Mateos, Avenida Wilfredo Massieu s/n, Zacatenco, Colonia Lindavista, CP 07738, México, DF, Mexico

Received 9 June 2014; Revised 7 August 2014; Accepted 9 August 2014; Published 3 September 2014

Academic Editor: Jairo Kenupp Bastos

Copyright © 2014 Isela Álvarez-González et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Blueberry is a plant with a number of nutritional and biomedical capabilities. In the present study we initially evaluated the capacity of its juice (BJ) to inhibit the number of aberrant crypts (AC) induced with azoxymethane (AOM) in mouse. BJ was administered daily by the oral route to three groups of animals during four weeks (1.6, 4.1, and 15.0 μL/g), respectively, while AOM (10 mg/kg) was intraperitoneally injected to the mentioned groups, twice a week, in weeks two and three of the assay. We also included two control groups of mice, one administered distilled water and the other the high dose of BJ. A significant increase of AC was observed in the AOM treated animals, and a mean protection of 75.6% was determined with the two low doses of BJ tested; however, the high dose of the juice administered together with AOM increased the number of crypts more than four times the value observed in animals administered only AOM. Furthermore, we determined the antioxidant potential of BJ with an ex vivo DPPH assay and found a dose-dependent decrease with a mean of 19.5%. We also determined the DNA oxidation/antioxidation by identifying 8-hydroxy-2′-deoxyguanosine adducts and found a mean decrease of 44.3% with the BJ administration with respect to the level induced by AOM. Our results show a complex differential effect of BJ related to the tested doses, opening the need to further evaluate a number of factors so as to determine the possibility of a cocarcinogenic potential.