TY - JOUR A2 - Lu, Aiping AU - Zhao, Linlin AU - Wan, Ling AU - Qiu, Xinjian AU - Li, Ruomeng AU - Liu, Shimi AU - Wang, Dongsheng PY - 2014 DA - 2014/07/20 TI - A Metabonomics Profiling Study on Phlegm Syndrome and Blood-Stasis Syndrome in Coronary Heart Disease Patients Using Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry SP - 385102 VL - 2014 AB - A metabonomics approach based on liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) was utilized to obtain potential biomarkers of coronary heart disease (CHD) patients and investigate the ZHENG types differentiation in CHD patients. The plasma samples of 20 CHD patients with phlegm syndrome, 20 CHD patients with blood-stasis syndrome, and 16 healthy volunteers were collected in the study. 26 potential biomarkers were identified in the plasma of CHD patients and 19 differential metabolites contributed to the discrimination of phlegm syndrome and blood-stasis syndrome in CHD patients (VIP>1.5; P<0.05) which mainly involved purine metabolism, pyrimidine metabolism, amino acid metabolism, steroid biosynthesis, and arachidonic acid metabolism. This study demonstrated that metabonomics approach based on LC-MS was useful for studying pathologic changes of CHD patients and interpreting the differentiation of ZHENG types (phlegm and blood-stasis syndrome) in traditional Chinese medicine (TCM). SN - 1741-427X UR - https://doi.org/10.1155/2014/385102 DO - 10.1155/2014/385102 JF - Evidence-Based Complementary and Alternative Medicine PB - Hindawi Publishing Corporation KW - ER -