Optimization of Cat’s Whiskers Tea (Orthosiphon stamineus) Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells

Table 4

Antiproliferative properties of the extracts of O. stamineus leaves obtained by supercritical CO₂.

Samples^a	IC50 μg/mL
	MCF-7	MDA-MB	HCT116	PC3	CCD-18 Co
A1	>200	>200	>200	>200	>200
A2	>200	>200	>200	>200	>200
A3	>200	>200	>200	>200	>200
B1	>200	>200	>200	84	>200
B2	100	57.8*	90	28*	93.08
B3	>200	>200	>200	>200	>200
C1	104	53*	NA	79*	94.5
C2	125	101	78*	78*	91.6
C3	92	90	80	73*	127
5FU	—	—	4.6**	—	—
Tamoxifen	9.5**	—	—	—	—
Betulinic acid	—	2.1**	8.4**	4.4**	12.9**

ND: not detected.
^aThe test for each extract was performed in triplicate and the results are presented as a mean percent inhibition ± SD. Each experiment was repeated three times ( = 3); * < 0.05; ** < 0.01.