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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 430757, 9 pages
http://dx.doi.org/10.1155/2014/430757
Research Article

PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury

1Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China
2Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China
3Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China
4Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China
5Buchang Pharmaceutical Co., Ltd., Xi’an 712000, China

Received 30 September 2013; Revised 13 January 2014; Accepted 17 January 2014; Published 23 February 2014

Academic Editor: Ching-Liang Hsieh

Copyright © 2014 Zefeng Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to investigate neuroprotection of Danhong injection (DHI) in a rat model of cerebral ischemia using 18F-fluorodeoxyglucose positron emission tomography (18F-FDG-PET). Method. Rats were divided into 5 groups: sham group, ischemia-reperfusion untreated (IRU) group, DHI-1 group (DHI 1 mL/kg/d), DHI-2 group (DHI 2 mL/kg/d), and DHI-4 group (DHI 4 mL/kg/d). AII the treated groups were intraperitoneally injected with DHI daily for 14 days. The therapeutic effects in terms of cerebral infarct volume, neurological function, and cerebral glucose metabolism were evaluated. Expression of TNF- and IL-1 was detected with enzyme-linked immunosorbent assay (ELISA). Levels of mature neuronal marker (NeuN), glial marker (GFAP), vascular density factor (vWF), and glucose transporter 1 (GLUT1) were assessed by immunohistochemistry. Results. Compared with the IRU group, rats treated with DHI showed dose dependent reductions in cerebral infarct volume and levels of proinflammatory cytokines, improvement of neurological function, and recovery of cerebral glucose metabolism. Meanwhile, the significantly increased numbers of neurons, gliocytes, and vessels and the recovery of glucose utilization were found in the peri-infarct region after DHI treatment using immunohistochemical analysis. Conclusion. This study demonstrated the metabolic recovery after DHI treatment by micro-PET imaging with 18F-FDG and the neuroprotective effects of DHI in a rat model of cerebral ischemic-reperfusion injury.