Immunomodulatory and Antidiabetic Effects of a New Herbal Preparation (HemoHIM) on Streptozotocin-Induced Diabetic Mice

Table 2

Cytokine and immunoglobulin production by spleen cells in mice administered HemoHIM.

	Nondiabetic	Nondiabetic + HemoHIM	Diabetic	Diabetic + HemoHIM
Cytokines (ng/mL)
IL-2	1.21 ± 0.22	1.21 ± 0.19	1.22 ± 0.29	1.22 ± 0.10
IFN-𝛾	6.61 ± 1.60^#	6.74 ± 2.37^#	2.85 ± 1.86*	5.01 ± 2.39
IL-6	1.03 ± 0.28^#	1.15 ± 0.30^#	1.57 ± 0.28*	0.90 ± 0.31^#
TNF-𝛼	0.39 ± 0.08	0.35 ± 0.15	0.53 ± 0.23	0.35 ± 0.08
Immunoglobulin (ng/mL)
IgM	616.0 ± 163.2	586.4 ± 93.4^#	873.5 ± 231.9	566.6 ± 290.6
IgG1	5.0 ± 2.7^#	5.2 ± 2.7	9.7 ± 3.6*	6.7 ± 1.2

The spleen cells (5×10⁶ cells/mL) were treated with Con A (1 𝜇g/mL) or LPS (10 𝜇g/mL) for 24 hours and culture supernatants were harvested and stored at −20°C until use. IL-2 and IFN-𝛾 were measured in the Con A-stimulated supernatant. IL-6, TNF-𝛼, IgM, and IgG1 were measured in the LPS-stimulated supernatant. Values are expressed as means ± S.D.
^∗𝑃<0.05 compared with nondiabetic group.
^#𝑃<0.05 compared with diabetic group.