Evidence-Based Complementary and Alternative Medicine / 2014 / Article / Tab 2

Research Article

Immunomodulatory and Antidiabetic Effects of a New Herbal Preparation (HemoHIM) on Streptozotocin-Induced Diabetic Mice

Table 2

Cytokine and immunoglobulin production by spleen cells in mice administered HemoHIM.

NondiabeticNondiabetic + HemoHIMDiabeticDiabetic + HemoHIM

Cytokines (ng/mL)
 IL-21.21 ± 0.221.21 ± 0.191.22 ± 0.291.22 ± 0.10
 IFN- 6.61 ± 1.60#6.74 ± 2.37#2.85 ± 1.86*5.01 ± 2.39
 IL-61.03 ± 0.28#1.15 ± 0.30#1.57 ± 0.28*0.90 ± 0.31#
 TNF- 0.39 ± 0.080.35 ± 0.150.53 ± 0.230.35 ± 0.08
Immunoglobulin (ng/mL)
 IgM616.0 ± 163.2586.4 ± 93.4#873.5 ± 231.9566.6 ± 290.6
 IgG15.0 ± 2.7#5.2 ± 2.79.7 ± 3.6*6.7 ± 1.2

The spleen cells ( cells/mL) were treated with Con A (1  g/mL) or LPS (10  g/mL) for 24 hours and culture supernatants were harvested and stored at −20°C until use. IL-2 and IFN- were measured in the Con A-stimulated supernatant. IL-6, TNF- , IgM, and IgG1 were measured in the LPS-stimulated supernatant. Values are expressed as means ± S.D.
compared with nondiabetic group.
compared with diabetic group.

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