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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 580483, 6 pages
Research Article

Cytotoxicity of Aporphine, Protoberberine, and Protopine Alkaloids from Dicranostigma leptopodum (Maxim.) Fedde

1Analytic and Testing Center, Beijing Normal University, No. 19 Xinjiekouwai Street, Haidian District, Beijing 100875, China
2College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning 116600, China
3State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, China
4Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Haidian District, Beijing 100875, China

Received 10 April 2014; Revised 2 May 2014; Accepted 2 May 2014; Published 21 May 2014

Academic Editor: Shi-Biao Wu

Copyright © 2014 Ruiqi Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nine alkaloids with three different structural skeletons were isolated from Dicranostigma leptopodum (Maxim.) Fedde (Papaveraceae) by repeated silica gel column chromatography. Their chemical structures were identified on the basic of physicochemical and spectroscopic data. Among them, 10-O-methylhernovine (1), nantenine (2), corytuberine (3), lagesianine A (4), and dihydrocryptopine (9) were first isolated from this plant. With a series of cytotoxic tests, compounds 2, 3, and 7 displayed cytotoxicity against SMMC-7721 with IC50 values of 70.08 ± 4.63, 73.22 ± 2.35, and 27.77 ± 2.29 μM, respectively.