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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 580745, 11 pages
Research Article

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC-DAD-MSn, and LC-DAD-ESI-IT-TOF/MS Analyses

1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Beijing 100700, China
3Jiangsu Kanion Pharmaceut Co. Ltd., Lianyungang 222001, China

Received 17 April 2014; Revised 28 June 2014; Accepted 30 June 2014; Published 20 July 2014

Academic Editor: Shi-Biao Wu

Copyright © 2014 Juanjuan Liang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chemical profiling is always the first task in the standardization and modernization of Traditional Chinese Medicine. HPLC and LC-MS were employed to find out the common chromatographic peaks in various batches of Tianshu Capsule (TSC) and the contribution of the characteristic peaks from individual herbs to the whole chromatographic profile of TSC sample. A total of 38 constituents were identified in TSC sample based on the comparison of retention time and UV spectra with authentic compounds as well as by summarized MS fragmentation rules and matching of empirical molecular formula with those of published components. This is the first systematic report on the chemical profiling of the commercial TSC product, which provides the sufficiently chemical evidence for the global quality evaluation of TSC products.