608721.fig.007a
(a)
608721.fig.007b
(b)
Figure 7: DBT stimulates transcriptional activity of estrogen responsive element and the HRE-mediated transcriptional activity. (a) Three repeats of estrogen responsive elements (ERE: 5′-GGT CAC AGT GAC C-3′) were subcloned into a luciferase-reporter vector called pERE-Luc (upper panel). This reporter was stably transfected to MCF-7 cells, which were treated with DBT extracts at different dose for 48 h (lower panel). 17-β-Estradiol (100 nM) was used as a positive control. (b) Six repeats of hypoxia responsive elements (HRE: 5′-TCG AGG CCC TAC GTG CTG TCT CAC ACA GCC TGT CTG ACG-3′) were subcloned in an expression vector and it was named as pHRE-Luc (upper panel). DBT extracts at different concentrations were applied to the pHRE-Luc-transfected HEK293T cells for 48 h; the promoter-driven luciferase (pHRE-Luc) activity was determined. DFO (50 μM) was used as positive controls. Values are expressed as the ratio to the basal reading where the control (untreated culture) is equal to 1 and are in Mean ± SD, where , each with triplicate samples. * .