Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases

Table 3

eNOSIDV/GAPDH IDV ratios in isolated rat aortic rings.

Treatment	Ratio
Phe (0.3 μM)	1.10 ± 0.08	5
Phe + ACh (10 μM)	1.24 ± 0.04	8
H2O2 (200 μM)	1.13 ± 0.03	8
TMP (10 nM ) + H2O2	1.01 ± 0.03	6
Losartan (1 μM ) + H2O2	1.13 ± 0.05	6
Endothelium removal	0.32 ± 0.04	6

Data represent the mean ± the SEM of 5–8 independent experiments.
ACh: acetylcholine; eNOS: endothelial nitric oxide synthase; GADPH: glyceraldehyde 3-phosphate dehydrogenase; H₂O₂: hydrogen peroxide; IDV: integrated density value; Phe: phenylephrine; TMP: tetramethylpyrazine.