Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt
The inhibitory effect of Morus alba leaves extracts (MAE) on platelet aggregation induced by collagen. Platelets (3 × 108/mL) were preincubated with or without MAE (100–400 μg/mL) in the presence of 1 mM CaCl2 for 2 min at 37°C. The platelet aggregation was then induced by 2.5 μg/mL collagen, 10 μM ADP, and 0.1 U/mL thrombin. The extent of platelet aggregation was measured with an aggregometer. The aggregation reaction was terminated after 5 min, and the percent aggregation rate was calculated. Each graph shows the mean ± SEM of at least four independent experiments. *** compared to the agonist control.