Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt
Effects of Morus alba leaves extracts (MAE) on collagen-induced phosphorylation of MAPKs, PI3K, and Akt. Washed platelets (3 × 108/mL) were pre-incubated for 2 min with vehicle or MAE at the indicated concentration. The platelets were then stimulated with 10 μM ADP for 5 min at 37°C. After terminating the reactions, total cell proteins were extracted. The proteins were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The membranes were then probed with antibodies against phospho-p44/42, p44/42, phospho-p38, p38, phospho-SAPK/JNK, and -actin ((a), (b)) and phospho-PI3K, phospho-Akt, PKAα/β/γ cat, and -actin ((c), (d)). Antibody binding was visualized by chemiluminescence. All immunoblots are representative of three or four independent experiments. ** and *** versus vehicle control.