Tanshinone IIA Downregulates HMGB1 and TLR4 Expression in a Spinal Nerve Ligation Model of Neuropathic Pain
Figure 2
(a) Transcript measured by quantitative real-time PCR showed significant differences in the HMGB1 gene level on the different postoperative days ( versus sham group, versus model group). (b) The HMGB1 protein measurement by western blotting showed that the HMGB1 (26 kDa) protein was at a low level in the sham group; the HMGB1 protein began to increase 3 days after SNL surgery and remained at a high level during the experiment ( versus sham group); after Tan IIA treatment, the HMGB1 protein reduced significantly ( versus model group). (c) Summary data of western blot showed significant differences in the HMGB1 protein level on different postoperative days ( versus sham group, versus model group). Data are shown as the mean ± S.D. SNL: spinal nerve ligation. HMGB1: high-mobility group box 1. Blank: sham group. Drug: Tan IIA group. S.D: standard deviation. Mean ± S.D.
