Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells

Figure 3

BRP ethanolic extract promotes apoptosis in 5637 cells. (a) Flow cytometry graphs for Annexin V-7AAD analysis of 5637 cells treated with 25, 50, and 100 μg/mL of BRP ethanolic extract for 24 h. Control group (A), 25 μg/mL group (B), 50 μg/mL group (C), and 100 μg/mL group (D). (b) Percentage of apoptotic cells after treatment with 25, 50, and 100 μg/mL of BRP ethanolic extract for 24 h. Data are expressed as means ± SEM from three independent experiments. Different letters (A, B, and C) indicate significant differences between the means and differences were considered significant at . (c) Selectivity of BFP in normal and tumoral cells after treatment with 100 μg/mL for 24 h. Data are expressed as means ± SEM from three independent experiments. Different letters (A, B, and C) indicate significant differences between the means and differences were considered significant at . () Early and late apoptosis together.

(a)

(b)

(c)