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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 640857, 9 pages
http://dx.doi.org/10.1155/2014/640857
Research Article

Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells

1Institute of Andrology, Nanjing University of Chinese Medicine, No. 138 Xianlin Road, Nanjing, Jiangsu 210023, China
2State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China

Received 8 January 2014; Revised 20 March 2014; Accepted 24 March 2014; Published 10 April 2014

Academic Editor: Yuping Tang

Copyright © 2014 Zhiqiang Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism. Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells. Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf. Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism. Results. After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly. The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells. Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract. Conclusion. YC extract could stimulate the proliferation of GC-1 spg cells. Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.