Alpha-Asarone Protects Endothelial Cells from Injury by Angiotensin II

Figure 2

Protection of α-asarone against changes in ROS/NO levels in EA.hy926 cells induced by Ang II. Cells were treated with serum-free and phenol red-free RPMI 1640 medium (Control group) or Ang II (Model group) or pretreated with 10, 50 and 100 μmol/L of α-asarone (α-A) for 1 h, followed by Ang II at 0.1 μmol/L for 24 h. The intracellular ROS and NO levels were detected with fluorescent dyes DHE and DAF-FM DA, respectively. Magnification, ×40 (a). Data are expressed as means ± SD from three independent experiments. ^∗∗𝑃<0.01 versus control; ^#𝑃<0.05 versus model; ^##𝑃<0.01 versus model (b).

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