TY - JOUR
A2 - Sirajudeen, Kuttulebbai N. S.
AU - Gong, Xiao-Gang
AU - Sun, Hong-Mei
AU - Zhang, Yi
AU - Zhang, Shu-Jing
AU - Gao, Yu-Shan
AU - Feng, Jing
AU - Hu, Jing-Hong
AU - Gai, Cong
AU - Guo, Zhen-Yu
AU - Xu, Hong
AU - Ma, Ling
PY - 2014
DA - 2014/12/24
TI - Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease
SP - 729195
VL - 2014
AB - Da-Bu-Yin-Wan (DBYW) and Qian-Zheng-San (QZS), two classic traditional Chinese medicinal formulas, were clinically employed to treat Parkinson’s disease (PD). Our previous studies demonstrated neuroprotective effects of them on mitochondrial function in PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The purpose of this research was to investigate their possible mechanisms in the light of mitochondrial ATP-sensitive potassium (mitoKATP) channels. The neuroprotective effect of DBYW and QZS on dopamine (DA) neurons in substantia nigra (SN) in the MPTP-induced PD mice was investigated by behavioral test (pole test) and immunohistochemistry. Adenosine triphosphate (ATP) level in the midbrain tissue was detected by firefly luciferase method. MitoKATP channel subunits SUR1 and Kir6.2 mRNA and protein expressions were tested by real-time PCR (RT-PCR) and Western blot. It was observed that DBYW and/or QZS served to ameliorate MPTP-induced behavioral impairment and prevent the loss of substantia nigra dopamine neurons, as well as increase ATP level in the midbrain tissue and downregulate SUR1 expression at mRNA and protein levels with no marked influence on Kir6.2. We concluded that DBYW and QZS exhibit neuroprotective effects probably through the regulation of ATP level and mitoKATP channel subunit expressions.
SN - 1741-427X
UR - https://doi.org/10.1155/2014/729195
DO - 10.1155/2014/729195
JF - Evidence-Based Complementary and Alternative Medicine
PB - Hindawi Publishing Corporation
KW -
ER -