Caffeamide 36-13 Regulates the Antidiabetic and Hypolipidemic Signs of High-Fat-Fed Mice on Glucose Transporter 4, AMPK Phosphorylation, and Regulated Hepatic Glucose Production

Figure 4

Semiquantitative RT-PCR analysis on PEPCK, G6Pase, SREBP1c, FAS, apo C-III, and adiponectin mRNA expression in liver tissue of the mice by oral gavage caffeamide 36-13 (TS) for 4 weeks. All values are means ± S.E. (𝑛=9). ^##𝑃<0.001, ^###𝑃<0.001 compared with the control (CON) group; ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001 compared with the high-fat + vehicle (HF) group. TS: T1: 10, T2: 20 mg/kg bodyweight; Rosi: rosiglitazone (0.01 g/kg body weight). Total RNA (1 μg) isolated from tissue was reverse transcripted by MMLV-RT, and 10 μL of RT products were used as templates for PCR. Signals were quantitated by image analysis; each value was normalized by GAPDH.

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