Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding

Table 2

Primers and PCR reaction conditions.

Primer name	Primer sequences (5′-3′)	PCR reaction condition
ITS2
2F	ATGCGATACTTGGTGTGAAT	94°C 5 min;
3R	GACGCTTCTCCAGACTACAAT	94°C 30 s, 56°C 30 s,; 72°C 45 s, 40 cycles; 72°C 10 min;
ITS
4R	TCCTCCGCTTATTGATATGC	94°C 5 min;
5F	GGAAGTAAAAGTCGTAACAAGG	94°C 1 min, 50°C 1 min,; 72°C 1.5 min + 3 s/cycle, 30 cycles; 72°C 7 min;
psbA
fwdPA	GTTATGCATGAACGTAATGCTC	94°C 4 min;
trnH
rev TH	CGCGCATGGTGGATTCACAATCC	94°C 30 s, 55°C 1 min,; 72°C 1 min, 35 cycles; 72°C 10 min;