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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 869831, 9 pages
Research Article

Cognitive Enhancing and Neuroprotective Effect of the Embryo of the Nelumbo nucifera Seed

1Biological and Genetic Resources Utilization Division, National Institute of Biological Resources, Hwangyeong-ro 42, Seo-gu, Incheon 404-708, Republic of Korea
2Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701, Republic of Korea
3Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea

Received 17 June 2014; Revised 24 November 2014; Accepted 25 November 2014; Published 24 December 2014

Academic Editor: Ki-Wan Oh

Copyright © 2014 Eun Sil Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the present study was to evaluate the effect of ENS on cognitive impairment induced by scopolamine and its potential neuroprotective effect against glutamate-induced cytotoxicity in HT22 cell and to investigate the underlying mechanisms. ENS (3, 10, 30, and 100 mg/kg), scopolamine (1 mg/kg), and donepezil (1 mg/kg) were administered to mice during a test period. Scopolamine impaired memory and learning in a water maze test and a passive avoidance test. The neuroprotective effect of ENS (10 and 100 μg/mL) was investigated on glutamate-induced cell death in HT22 cells by MTT assay. We investigated acetylcholinesterase inhibition in hippocampus and antioxidant activity, ROS levels, and Ca2+ influx in HT22 cells to elucidate the potential mechanisms of ENS. We found that ENS significantly ameliorated scopolamine-induced memory impairment and inhibited AChE activity in hippocampus. In vitro, ENS showed potent neuroprotective effects against glutamate-induced neurotoxicity in the HT22 cell. In addition, ENS induced a decrease in ROS production and intercellular Ca2+ accumulation and showed DPPH radical and H2O2 scavenging activity. In conclusion, ENS showed both a memory improving effect and a neuroprotective effect. Our results indicate that ENS may be of use in the treatment and prevention of neurodegenerative disorders.