Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 895272, 11 pages
http://dx.doi.org/10.1155/2014/895272
Research Article

Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes

1Department of Clinical Nursing Science, Yonsei University College of Nursing, Seoul 120-752, Republic of Korea
2Nursing Policy and Research Institute, Biobehavioral Research Center, Yonsei University, Seoul 120-752, Republic of Korea
3Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea
4Department of Emergency Medical Technology, Kyungil Univeristy, Gyeongsan, Kyungbook 712-701, Republic of Korea
5Sport and Medicine Research Center, INTOTO Inc., 401 Dawoo BD, 90-6 Daeshin-Dong, Seodaemun-Gu, Seoul 120-160, Republic of Korea

Received 20 March 2014; Revised 19 August 2014; Accepted 19 August 2014; Published 11 September 2014

Academic Editor: Pradeep Visen

Copyright © 2014 Jae-Yeo Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polyphenols have recently become an important focus of study in obesity research. Oligonol is an oligomerized polyphenol, typically comprised of catechin-type polyphenols from a variety of fruits, which has been found to exhibit better bioavailability and bioreactivity than natural polyphenol compounds. Here, we demonstrated that Oligonol inhibits 3T3-L1 adipocyte differentiation by reducing adipogenic gene expression. During adipogenesis, Oligonol downregulated the mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and δ (C/EBPδ) in a dose-dependent manner and the expression of genes involved in lipid biosynthesis. The antiadipogenic effect of Oligonol appears to originate from its ability to inhibit the Akt and mammalian target of rapamycin (mTOR) signaling pathway by diminishing the phosphorylation of ribosomal protein S6 kinase (p70S6K), a downstream target of mTOR and forkhead box protein O1 (Foxo1). These results suggest that Oligonol may be a potent regulator of obesity by repressing major adipogenic genes through inhibition of the Akt signaling pathway, which induces the inhibition of lipid accumulation, ultimately inhibiting adipogenesis.