Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes
Figure 3
Effect of Oligonol on the expression of adipogenesis-related genes during 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated into adipocytes in the absence or presence of Oligonol (10, 25, or 50 μg/mL) for 8 days. (a) Oligonol inhibited the mRNA expressions of aP2, GLUT4, and Adiponectin during differentiation. The gene expressions were analyzed by quantitative RT-PCR. All gene expressions were normalized using GAPDH as a reference gene. (b) Protein expression levels of aP2, GLUT4, and adiponectin were analyzed by Western blot analysis in 3T3-L1 adipocytes treated with Oligonol. Cell lysates were collected from 3T3-L1 cells at days 4 and 8 after induction of adipocyte differentiation. Values are mean ± SEM of three independent experiments carried out in triplicates. *, **, *** compared with untreated adipocytes at each gene expression.
