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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 912450, 7 pages
Research Article

Protective Effect of Solanum nigrum Leaves Extract on Immobilization Stress Induced Changes in Rat’s Brain

1Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia
2Department of Neurology, Georgia Regents University, Augusta, GA 30912, USA
3King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
4Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
5Department of Surgery, Burn and Shock Trauma Research Institute, Loyola University, 1032 W. Sheridan Road, Chicago, IL 60660, USA
6Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
7College of Medical Rehabilitation, Qassim University, Buraydah, Saudi Arabia

Received 12 November 2013; Revised 18 December 2013; Accepted 18 December 2013; Published 9 February 2014

Academic Editor: Andrew Newberg

Copyright © 2014 Syed Kashif Zaidi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The prophylactic or curative antioxidant efficacy of crude extract and the active constituent of S. nigrum leaves were evaluated in modulating inherent antioxidant system altered due to immobilization stress in rat brain tissues, in terms of measurement of glutathione (GSH), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and free radical scavenging enzymes activities. Rats were treated with single dose of crude extract of S. nigrum prior to and after 6 h of immobilization stress exposure. Exposure to immobilization stress resulted in a decrease in the brain levels of glutathione, SOD, GST, and catalase, with an increase in thiobarbituric acid reactive substances (TBARS) levels. Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter. Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content. Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.