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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 924560, 11 pages
http://dx.doi.org/10.1155/2014/924560
Research Article

Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells

1Department of Physical Medicine and Rehabilitation, School of Medicine, College of Medicine, Taipei Medical University (TMU), No. 250, Wuxing Street, Taipei 11031, Taiwan
2Department of Physical Medicine and Rehabilitation, Taipei Medical University Hospital (TMUH), No. 252, Wuxing Street, Taipei 11031, Taiwan
3International Institute of Nano Biomedicine, TMU, No. 252, Wuxing Street, Taipei 11031, Taiwan
4Graduate Institute of Medical Sciences, College of Medicine, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
5Center for Teeth Bank and Dental Stem Cell Technology, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
6Department of Urology, School of Medicine, College of Medicine, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
7Department of Urology, TMUH, No. 252, Wuxing Street, Taipei 11031, Taiwan

Received 8 October 2013; Revised 5 January 2014; Accepted 22 January 2014; Published 26 March 2014

Academic Editor: Yew-Min Tzeng

Copyright © 2014 Chien-Hung Lai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Osteoblast differentiation plays an essential role in bone integrity. Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is available. This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells. The results showed that 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F) stimulated ALP activity. However, baicalein and luteolin inhibited ALP activity and flavone showed no effect. Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells. Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB), extracellular signal-regulated kinases (ERK 1/2), and the c-Jun N-terminal kinase (JNK) signaling pathways. On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways. Finally, after 5 weeks of 6-OH-F induction, MC3T3-E1 cells showed a significant increase in the calcein staining intensity relative to merely visible mineralization observed in cells cultured in the osteogenic medium only. These results suggested that 6-OH-F could activate AKT, ERK 1/2, and JNK signaling pathways to effectively promote osteoblastic differentiation.