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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 924560, 11 pages
http://dx.doi.org/10.1155/2014/924560
Research Article

Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells

1Department of Physical Medicine and Rehabilitation, School of Medicine, College of Medicine, Taipei Medical University (TMU), No. 250, Wuxing Street, Taipei 11031, Taiwan
2Department of Physical Medicine and Rehabilitation, Taipei Medical University Hospital (TMUH), No. 252, Wuxing Street, Taipei 11031, Taiwan
3International Institute of Nano Biomedicine, TMU, No. 252, Wuxing Street, Taipei 11031, Taiwan
4Graduate Institute of Medical Sciences, College of Medicine, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
5Center for Teeth Bank and Dental Stem Cell Technology, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
6Department of Urology, School of Medicine, College of Medicine, TMU, No. 250, Wuxing Street, Taipei 11031, Taiwan
7Department of Urology, TMUH, No. 252, Wuxing Street, Taipei 11031, Taiwan

Received 8 October 2013; Revised 5 January 2014; Accepted 22 January 2014; Published 26 March 2014

Academic Editor: Yew-Min Tzeng

Copyright © 2014 Chien-Hung Lai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. J. Boyle, W. S. Simonet, and D. L. Lacey, “Osteoclast differentiation and activation,” Nature, vol. 423, no. 6937, pp. 337–342, 2003. View at Publisher · View at Google Scholar · View at Scopus
  2. A. M. Parfitt, “Bone remodeling and bone loss: understanding the pathophysiology of osteoporosis,” Clinical Obstetrics and Gynecology, vol. 30, no. 4, pp. 789–811, 1987. View at Google Scholar · View at Scopus
  3. A. C. Feldstein, D. Weycker, G. A. Nichols et al., “Effectiveness of bisphosphonate therapy in a community setting,” Bone, vol. 44, no. 1, pp. 153–159, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. B. A. Lenart, D. G. Lorich, and J. M. Lane, “Atypical fractures of the femoral diaphysis in postmenopausal women taking alendronate,” The New England Journal of Medicine, vol. 358, no. 12, pp. 1304–1306, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. A. S. Neviaser, J. M. Lane, B. A. Lenart, F. Edobor-Osula, and D. G. Lorich, “Low-energy femoral shaft fractures associated with alendronate use,” Journal of Orthopaedic Trauma, vol. 22, no. 5, pp. 346–350, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Schmidt, “The 2012 hormone therapy position statement of the North American Menopause Society,” Menopause, vol. 19, no. 3, pp. 257–271, 2012. View at Google Scholar · View at Scopus
  7. W. Y. Liao, C. C. Liaw, Y. C. Huang et al., “Cyclohexylmethyl flavonoids suppress propagation of breast cancer stem cells via downregulation of NANOG,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 170261, 14 pages, 2013. View at Publisher · View at Google Scholar
  8. E. Middleton Jr., C. Kandaswami, and T. C. Theoharides, “The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer,” Pharmacological Reviews, vol. 52, no. 4, pp. 673–751, 2000. View at Google Scholar · View at Scopus
  9. K. Wang, S. Ping, S. Huang et al., “Molecular mechanisms underlying the in vitro anti-inflammatory effects of a flavonoid-rich ethanol extract from Chinese propolis (poplar type),” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 127672, 11 pages, 2013. View at Publisher · View at Google Scholar
  10. K. R. Narayana, M. S. Reddy, M. R. Chaluvadi, and D. R. Krishna, “Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential,” Indian Journal of Pharmacology, vol. 33, no. 1, pp. 2–16, 2001. View at Google Scholar · View at Scopus
  11. G. Zhang, L. Qin, and Y. Shi, “Epimedium-derived phytoestrogen flavonoids exert beneficial effect on preventing bone loss in late postmenopausal women: a 24-month randomized, double-blind and placebo-controlled trial,” Journal of Bone and Mineral Research, vol. 22, no. 7, pp. 1072–1079, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. V. G. Palacios, L. J. Robinson, C. W. Borysenko, T. Lehmann, S. E. Kalla, and H. C. Blair, “Negative regulation of RANKL-induced osteoclastic differentiation in RAW264.7 cells by estrogen and phytoestrogens,” The Journal of Biological Chemistry, vol. 280, no. 14, pp. 13720–13727, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. C. K. Park, Y. Lee, E.-J. Chang et al., “Bavachalcone inhibits osteoclast differentiation through suppression of NFATc1 induction by RANKL,” Biochemical Pharmacology, vol. 75, no. 11, pp. 2175–2182, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. Y. W. Wu, S. C. Chen, W. F. Lai, Y. C. Chen, and Y. H. Tsai, “Screening of flavonoids for effective osteoclastogenesis suppression,” Analytical Biochemistry, vol. 433, no. 1, pp. 48–55, 2013. View at Publisher · View at Google Scholar
  15. X. Wang, L. Zhen, G. Zhang, M.-S. Wong, L. Qin, and X. Yao, “Osteogenic effects of flavonoid aglycones from an osteoprotective fraction of Drynaria fortunei—an in vitro efficacy study,” Phytomedicine, vol. 18, no. 10, pp. 868–872, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. A. J. Y. Guo, R. C. Y. Choi, A. W. H. Cheung et al., “Baicalin, a flavone, induces the differentiation of cultured osteoblasts: an action via the Wnt/β-catenin signaling pathway,” The Journal of Biological Chemistry, vol. 286, no. 32, pp. 27882–27893, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. L.-H. Chiu, S.-C. Chen, K.-C. Wu et al., “Differential effect of ECM molecules on re-expression of cartilaginous markers in near quiescent human chondrocytes,” Journal of Cellular Physiology, vol. 226, no. 8, pp. 1981–1988, 2011. View at Publisher · View at Google Scholar · View at Scopus
  18. L. D. Quarles, D. A. Yohay, L. W. Lever, R. Caton, and R. J. Wenstrup, “Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development,” Journal of Bone and Mineral Research, vol. 7, no. 6, pp. 683–692, 1992. View at Google Scholar · View at Scopus
  19. A. Chin, Y. Yang, L. Chai, R. W. K. Wong, and A.-B. M. Rabie, “Effects of medicinal herb salvia miltiorrhiza on osteoblastic cells in vitro,” Journal of Orthopaedic Research, vol. 29, no. 7, pp. 1059–1063, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. G. A. Rodan, “Introduction to bone biology,” Bone, vol. 13, supplement 1, pp. S3–S6, 1992. View at Google Scholar · View at Scopus
  21. Y. J. Chen, C. S. Wu, J. J. Shieh et al., “Baicalein triggers mitochondria-mediated apoptosis and enhances the antileukemic effect of vincristine in childhood acute lymphoblastic leukemia CCRF-CEM cells,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 124747, 19 pages, 2013. View at Publisher · View at Google Scholar
  22. M. Li-Weber, “New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin,” Cancer Treatment Reviews, vol. 35, no. 1, pp. 57–68, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. G. Seelinger, I. Merfort, and C. M. Schempp, “Anti-oxidant, anti-inflammatory and anti-allergic activities of luteolin,” Planta Medica, vol. 74, no. 14, pp. 1667–1677, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Selvendiran, H. Koga, T. Ueno et al., “Luteolin promotes degradation in signal transducer and activator of transcription 3 in human hepatoma cells: an implication for the antitumor potential of flavonoids,” Cancer Research, vol. 66, no. 9, pp. 4826–4834, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Y. Wu, K. W. Tsai, Y. Z. Li et al., “Anti-bladder-tumor effect of Baicalein from Scutellaria baicalensis Georgi and its application in vivo,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 579751, 12 pages, 2013. View at Publisher · View at Google Scholar
  26. J. M. Kim, S.-U. Lee, Y. S. Kim, Y. K. Min, and S. H. Kim, “Baicalein stimulates osteoblast differentiation via coordinating activation of MAP kinases and transcription factors,” Journal of Cellular Biochemistry, vol. 104, no. 5, pp. 1906–1917, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. H. Yang, Q. Liu, J. H. Ahn et al., “Luteolin downregulates IL-1β-induced MMP-9 and-13 expressions in osteoblasts via inhibition of ERK signalling pathway,” Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 27, no. 2, pp. 261–266, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. R. A. Hipskind and G. Bilbe, “MAP kinase signaling cascades and gene expression in osteoblasts,” Frontiers in Bioscience, vol. 3, pp. d804–d816, 1998. View at Google Scholar · View at Scopus
  29. C.-F. Lai, L. Chaudhary, A. Fausto et al., “Erk is essential for growth, differentiation, integrin expression, and cell function in human osteoblastic cells,” The Journal of Biological Chemistry, vol. 276, no. 17, pp. 14443–14450, 2001. View at Publisher · View at Google Scholar · View at Scopus
  30. O. Wanachewin, K. Boonmaleerat, P. Pothacharoen, V. Reutrakul, and P. Kongtawelert, “Sesamin stimulates osteoblast differentiation through p38 and ERK1/2 MAPK signaling pathways,” BMC Complementary & Alternative Medicine, vol. 12, article 71, 2012. View at Google Scholar
  31. Y.-X. Yan, Y.-W. Gong, Y. Guo et al., “Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation,” PLoS ONE, vol. 7, no. 4, Article ID e35709, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. M. B. Greenblatt, J. H. Shim, and L. H. Glimcher, “Mitogen-activated protein kinase pathways in osteoblasts,” Annual Review of Cell and Developmental Biology, vol. 29, pp. 63–79, 2013. View at Publisher · View at Google Scholar
  33. G. Xiao, D. Jiang, R. Gopalakrishnan, and R. T. Franceschi, “Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2,” The Journal of Biological Chemistry, vol. 277, no. 39, pp. 36181–36187, 2002. View at Publisher · View at Google Scholar · View at Scopus
  34. G. Xiao, D. Jiang, P. Thomas et al., “MAPK pathways activate and phosphorylate the osteoblast-specific transcription factor, Cbfa1,” The Journal of Biological Chemistry, vol. 275, no. 6, pp. 4453–4459, 2000. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Guicheux, J. Lemonnier, C. Ghayor, A. Suzuki, G. Palmer, and J. Caverzasio, “Activation of p38 mitogen-activated protein kinase and c-Jun-NH2-terminal kinase by BMP-2 and their implication in the stimulation of osteoblastic cell differentiation,” Journal of Bone and Mineral Research, vol. 18, no. 11, pp. 2060–2068, 2003. View at Publisher · View at Google Scholar · View at Scopus
  36. Y. S. Hah, H. G. Kang, H. Y. Cho et al., “JNK signaling plays an important role in the effects of TNF-α and IL-1β on in vitro osteoblastic differentiation of cultured human periosteal-derived cells,” Molecular Biology Reports, vol. 40, no. 8, pp. 4869–4881, 2013. View at Publisher · View at Google Scholar
  37. T. Matsuguchi, N. Chiba, K. Bandow, K. Kakimoto, A. Masuda, and T. Ohnishi, “JNK activity is essential for Atf4 expression and late-stage osteoblast differentiation,” Journal of Bone and Mineral Research, vol. 24, no. 3, pp. 398–410, 2009. View at Publisher · View at Google Scholar · View at Scopus
  38. N. Kawamura, F. Kugimiya, Y. Oshima et al., “Akt1 in osteoblasts and osteoclasts controls bone remodeling,” PLoS ONE, vol. 2, no. 10, Article ID e1058, 2007. View at Publisher · View at Google Scholar · View at Scopus
  39. A. Mukherjee, E. M. Wilson, and P. Rotwein, “Selective signaling by Akt2 promotes bone morphogenetic protein 2-mediated osteoblast differentiation,” Molecular and Cellular Biology, vol. 30, no. 4, pp. 1018–1027, 2010. View at Publisher · View at Google Scholar · View at Scopus
  40. F. Kugimiya, H. Kawaguchi, S. Ohba et al., “GSK-3β controls osteogenesis through regulating Runx2 activity,” PLoS ONE, vol. 2, no. 9, Article ID e837, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. S. Patel, B. Doble, and J. R. Woodgett, “Glycogen synthase kinase-3 in insulin and Wnt signalling: a double-edged sword?” Biochemical Society Transactions, vol. 32, no. 5, pp. 803–808, 2004. View at Publisher · View at Google Scholar · View at Scopus
  42. W. Zhao, M. H. Byrne, Y. Wang, and S. M. Krane, “Osteocyte and osteoblast apoptosis and excessive bone deposition accompany failure of collagenase cleavage of collagen,” The Journal of Clinical Investigation, vol. 106, no. 8, pp. 941–949, 2000. View at Google Scholar · View at Scopus
  43. M. Bosetti, M. Leigheb, R. A. Brooks, F. Boccafoschi, and M. F. Cannas, “Regulation of osteoblast and osteoclast functions by FGF-6,” Journal of Cellular Physiology, vol. 225, no. 2, pp. 466–471, 2010. View at Publisher · View at Google Scholar · View at Scopus
  44. L. Ren, F. Wang, Z. Xu, W. M. Chan, C. Zhao, and H. Xue, “GABAA receptor subtype selectivity underlying anxiolytic effect of 6-hydroxyflavone,” Biochemical Pharmacology, vol. 79, no. 9, pp. 1337–1344, 2010. View at Publisher · View at Google Scholar · View at Scopus
  45. S.-U. Choi, S. Y. Ryu, S.-K. Yoon et al., “Effects of flavonoids on the growth and cell cycle of cancer cells,” Anticancer Research, vol. 19, no. 6, pp. 5229–5233, 1999. View at Google Scholar · View at Scopus
  46. W. Herath, J. R. Mikell, A. L. Hale, D. Ferreira, and I. A. Khan, “Microbial metabolism—part 9. Structure and antioxidant significance of the metabolites of 5,7-dihydroxyflavone (chrysin), and 5- and 6-hydroxyflavones,” Chemical and Pharmaceutical Bulletin, vol. 56, no. 4, pp. 418–422, 2008. View at Publisher · View at Google Scholar · View at Scopus
  47. M. S. Y. Huen, K.-M. Hui, J. W. C. Leung et al., “Naturally occurring 2′-hydroxyl-substituted flavonoids as high-affinity benzodiazepine site ligands,” Biochemical Pharmacology, vol. 66, no. 12, pp. 2397–2407, 2003. View at Publisher · View at Google Scholar · View at Scopus
  48. S. Bandyopadhyay, J.-M. Lion, R. Mentaverri et al., “Attenuation of osteoclastogenesis and osteoclast function by apigenin,” Biochemical Pharmacology, vol. 72, no. 2, pp. 184–197, 2006. View at Publisher · View at Google Scholar · View at Scopus
  49. E.-M. Choi, “Apigenin increases osteoblastic differentiation and inhibits tumor necrosis factor-α-induced production of interleukin-6 and nitric oxide in osteoblastic MC3T3-E1 cells,” Die Pharmazie, vol. 62, no. 3, pp. 216–220, 2007. View at Publisher · View at Google Scholar · View at Scopus