Proteomic Identification of Nrf2-Mediated Phase II Enzymes Critical for Protection of Tao Hong Si Wu Decoction against Oxygen Glucose Deprivation Injury in PC12 Cells

Figure 6

Role ofNrf2-mediated phase II enzymes in the protective effect of THSWD against OGD-Rep induced injury in PC12 cells. Cells were separately treated with two concentrations of THSWD (0.5 and 1.0 mg·mL⁻¹) with and without ARE decoy ODNs or mut ODNs pretreatment. Subsequently, the cells were subjected to OGD for 8 h and finally maintained in oxygenated cell culture medium for another 24 h. The cell viability was determined by CCK-8 assay. Values represent mean ± SD (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001.