Antiviral Activity of Total Flavonoid Extracts from Selaginella moellendorffii Hieron against Coxsackie Virus B3 In Vitro and In Vivo

Table 1

Cytotoxicity (CC50: μg/mL), in vitro antiviral activity (IC50: μg/mL), and therapeutic index (TI) for each sample.

Samples	CC50^a	Treating before infection		Treating during infection		Treating after infection
		IC50^a	TI	IC50^a	TI	IC50^a	TI
TFE	85 ± 1.7	NA	NA	41 ± 1.2	2.07	19 ± 1.6	4.47
EAE	60 ± 2.1	NA	NA	36 ± 1.9	1.67	16 ± 1.9	3.75
Amentoflavone	53 ± 0.9	NA	NA	52 ± 0.8	1.02	25 ± 1.2	2.12
ACV	>500	NA	NA	NA	NA	NA	NA

CC50: the half of the cytotoxic concentration; IC50: the inhibitory concentration required to reduce viral replication by 50%; TI: the ratio of CC50/IC50; NA: no activity in the assayed concentrations; ACV: acyclovir; EAE: ethyl acetate extracts of S. moellendorffii; TFE: total flavonoid extracts of S. moellendorffii. aMean ± SD values are shown from three independent experiments.