The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma
Figure 7
Effects of MMDT treatment on airway remodeling in lung tissue (MLC2 immunohistochemistry). (a) Stainedmouse lung sections and (b) MLC2 area (m2). Mouse lung sections were stained for MLC2 detection. The sections were incubated at 4°C overnight with an anti-MLC2 goat polyclonal antibody (1 : 50). The slides were then incubated with avidin-biotin peroxidase complex, and color was developed using 3,3-diaminobenzidine tetrachloride. The arrows indicate MCL2 positive cells (magnification 200x). Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 100 mg/kg of MMDT (MMDT 100 mg/kg), CKA-challenged mice treated with 200 mg/kg of MMDT (MMDT 200 mg/kg), and CKA-challenged mice treated with 400 mg/kg of MMDT (MMDT 400 mg/kg). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, **, ***; significantly different from CKA, ##, ###, -7).
