A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats
Figure 5
Effects of acupuncture on increasing population of immunopositive cells in penumbra: 5 visual regions in penumbra of each slice were selected for cell counting under a 400x microscope (). (a) Representative images of Immunofluorescence double-staining from model group, no-acupuncture group, and acupuncture group: a1, b1, and c1 labeled for BrdU (green). a2, b2, and c2 labeled for nestin (red). a3, b3, and c3 colabeled for BrdU/nestin. White arrows points to the BrdU/nestin+ cells. (b), (c), and (d) Quantization of BrdU+, nestin+, and BrdU/nestin+ cells: immunopositive cells were found in MCAO groups while not been found in normal and sham-operated group. Acupuncture enhanced BrdU+, nestin+, and BrdU/nestin+ labeled cells proliferation (▲, acupuncture group versus model group and no-acupuncture group). More BrdU+ labeled cells in no-acupuncture group than model group (★).
