Olive Leaf Extract Attenuates Obesity in High-Fat Diet-Fed Mice by Modulating the Expression of Molecules Involved in Adipogenesis and Thermogenesis
Effects of OLE on genes regulating adipogenesis in the epididymal adipose tissue of HFD-fed mice. (a) Gene expression of SFRP5, WNT10b, and LRP5, (b) protein levels of β-catenin, (c) mRNA expression of genes involved in the galanin-mediated signaling pathway, (d) protein levels of p-ERK and total ERK, (e) expression of PPARγ2, C/EBPα, and their target genes. Reverse transcriptase-polymerase chain reaction (RT-PCR) data represent the relative density normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Protein levels were normalized to the β-actin level. Data represent the results of three independent experiments ( or 3 per experiment); indicates statistical significance. Values are the means ± SEM, for each group.