Antidiabetic and Antihyperlipidemic Effects of Clitocybe nuda on Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in High-Fat-Fed Mice

Figure 5

The phospho-AMPK (Thr172) protein contents in (a) liver, (b) skeletal muscle, and (c) white adipose tissue and GLUT4 protein contents in (d) skeletal muscle and (e) adipose tissue of the mice by oral gavage extract of Clitocybe nuda for 4 weeks. Protein was separated by 12% SDS-PAGE detected by western blot. All values are mean ± S.E. (𝑛=9). ^#𝑃<0.05 and ^##𝑃<0.01 were compared with the control (CON) group; ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 were compared with the high-fat + vehicle (distilled water) (HF) group. C1, C2, and C3: extract of Clitocybe nuda (C1: 0.2, C2: 0.5, and C3: 1.0 g/kg bodyweight); Rosi: rosiglitazone (0.01 g/kg body weight).

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