Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through -Mediated Pathway
Figure 2
HFBF induced the secretion of GLP-1 in enteroendocrine cells without cell damage. BF extracted was fractionated with -hexane (HX). (a) HFBF was treated with 100, 200, and 500 g/mL each to the differentiated NCI-H716 cells. GLP-1 secretion was compared with the negative control and positive control. 1% DMSO used as negative control and 2 mM Quinine (Q) used as positive control. Experiments were conducted in triplicate and normalized with protein contents. (b) The effect of HFBF on the cell viability was measured by MTT test. All concentrations of HFBF had no toxicity to the NCI-H716 cell. Statistical significance was determined by a one-way ANOVA and the values are means ± SEM; , , .
