Figure 3: Histological analyses of cranial tibial muscle using HE staining (a–d). In (c), inside the circle, centralized nuclei and increased heterogeneity in myofibers size, characteristics of mdx muscle degeneration. Dystrophin expression in tibial cranial muscles after 52 days of the treatment (e–i). Groups: SHED/false acupoints; saline/true acupoints; SHED/true acupoints; and control. Scale Bar—100 μm.