A Cistanches Herba Fraction/β-Sitosterol Causes a Redox-Sensitive Induction of Mitochondrial Uncoupling and Activation of Adenosine Monophosphate-Dependent Protein Kinase/Peroxisome Proliferator-Activated Receptor γ Coactivator-1 in C2C12 Myotubes: A Possible Mechanism Underlying the Weight Reduction Effect

Figure 3

Time-course of HCF1-induced changes in AMPK activation in C2C12 myotubes. Cells were preincubated with HCF1 for increasing periods of time at 37°C. The extent of AMPK activation was assessed as described in Materials and Methods section. Data are expressed as percent control with respect to non-HCF1 preincubated time-match controls. Values given are means ± SEM, with . ^*Significantly different from non-drug preincubated time-match controls ().

(a)

(b)