A Cistanches Herba Fraction/β-Sitosterol Causes a Redox-Sensitive Induction of Mitochondrial Uncoupling and Activation of Adenosine Monophosphate-Dependent Protein Kinase/Peroxisome Proliferator-Activated Receptor γ Coactivator-1 in C2C12 Myotubes: A Possible Mechanism Underlying the Weight Reduction Effect

Figure 8

Effects of HCF1 on mitochondrial UCP3 and COX expression in C2C12 myotubes. Cells were preincubated with HCF1 for 48 h. The levels of mitochondrial UCP3 and COX were measured by Western blot analysis. Data are expressed as percent control with respect to non-drug preincubated controls. Values given were means ± SEM, with . ^*Significantly different from unpreincubated control groups; ^#significantly different from the CC preincubated, HCF1 unpreincubated control group ().

(a)

(b)

(c)

(d)