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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 145140, 13 pages
http://dx.doi.org/10.1155/2015/145140
Research Article

Improvement in Long-Term Memory following Chronic Administration of Eryngium planum Root Extract in Scopolamine Model: Behavioral and Molecular Study

1Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland
2Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
3Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland
4Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland
5Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland
6Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland
7Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland
8Laboratory of Molecular Biology, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland
9Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland
10Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

Received 15 February 2015; Revised 1 June 2015; Accepted 15 June 2015

Academic Editor: Armando Zarrelli

Copyright © 2015 Marcin Ozarowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Eryngium planum L. (EP) is as a rare medicinal plant with a lot of potentials as pharmaceutical crops. The aim of our study was to assess the effect of subchronic (28-fold) administration of a 70% ethanol extract of EP roots (200 mg/kg, p.o.) on behavioral and cognitive responses in Wistar rats linked with acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and beta-secretase (BACE-1) mRNA levels and AChE and BuChE activities in the hippocampus and frontal cortex. On the last day of experiment, 30 min after the last dose of EP or Huperzine A (HU), scopolamine (SC) was given at a dose of 0.5 mg/kg b.w. intraperitoneally. The results of a passive avoidance test showed an improvement in long-term memory produced by the EP extract in both scopolamine-induced rats and control group. EP caused an insignificant inhibition of AChE and BuChE activities in the frontal cortex and the hippocampus. EP decreased mRNA AChE, BuChE, and BACE-1 levels, especially in the cortex. Our results suggest that the EP extract led to the improvement of the long-term memory in rats coupled with total saponin content. The mechanism of EP action is probably complicated, since HPLC-MS analysis showed 64 chemical compounds (phenolics, saponins) in the extract of EP roots.