Evidence-Based Complementary and Alternative Medicine

Research Article

Germinated Brown Rice Alters Aβ(1-42) Aggregation and Modulates Alzheimer’s Disease-Related Genes in Differentiated Human SH-SY5Y Cells

Table 1

Gene names, accession numbers, and reverse and forward primer sequences used in GeXP Multiplex Gene Expression Analysis.
Gene nameAccession numberPrimer sequences with universal tags (underlined)
ForwardReverse
ACTBNM_001101AGGTGACACTATAGAATAGATCATTGCTCCTCCTGAGCGTACGACTCACTATAGGGAAAAGCCATGCCAATCTCATC
ADAM10NM_001110AGGTGACACTATAGAATACTAAACCAAACTTCACAGACTGTACGACTCACTATAGGGATTCTTCATACAGCATACATGG
APPNM_000484AGGTGACACTATAGAATAGGTTTGGCACTGCTCGTACGACTCACTATAGGGACATTCATGTGCATGTTC
BACE1NM_001207048AGGTGACACTATAGAATATTGAAGCTGCAGTCAAATGTACGACTCACTATAGGGAATGGCAAACTTGTAACAGTC
BACE2NM_012105AGGTGACACTATAGAATAGGCCTGAATTATGAATGTTAGTACGACTCACTATAGGGAGCATAGGACACAATCCAC
EEF1A1NM_001402AGGTGACACTATAGAATACACACGGCTCACATTGCATGTACGACTCACTATAGGGACACGAACAGCAAAGCGA
GAPDHNM_002046AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAAGTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG
GlucuronidaseNM_000181AGGTGACACTATAGAATAGGTTGGAGAGCTCATTTGGAGTACGACTCACTATAGGGAGAACAGTCCAGGAGGCACTT
KanR—AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTGGTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC
LRP1NM_002332AGGTGACACTATAGAATAATCGATCTTCACAAAGGAGGTACGACTCACTATAGGGAATCTGATCCAGGTTACTCTC
NeprilysinNM_000902AGGTGACACTATAGAATACCTTAGCAATTAAAGAAAGG GTACGACTCACTATAGGGAGTTGTTTACTTTGGCTGAAT
Presenilin 1NM_000021AGGTGACACTATAGAATATACTTCCAGAATGCACAGAGTACGACTCACTATAGGGATTCTTCCTCATCTTGCTC
Presenilin 2NM_000447AGGTGACACTATAGAATAGGAAGAAGTGTGTGATGAGGTACGACTCACTATAGGGAGTATTTGAGGGTCAGCTCT
Based on the Homo sapiens gene sequences adopted from the National Center for Biotechnology Information GenBank Database. Housekeeping genes; internal control; #normalization gene. ACTB: beta-actin; ADAM: a disintegrin and metalloproteinase; APP: amyloid precursor protein; BACE: beta-site APP-cleaving enzyme; EEF1A1: eukaryotic translation elongation factor 1 alpha 1; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; LRP1: low density lipoprotein receptor-related protein 1.