In Vitro and In Vivo Antitumor Effects of n-Butanol Extracts of Pterocephalus hookeri on Hep3B Cancer Cell

Figure 2

YSC-ZDC inhibited the proliferation of Hep3B cells. (a) YSC-ZDC inhibited cell proliferation in Hep3B cells. Cells were treated with YSC-ZDC (0–500 μg/mL) or 5-Fu (0–40 μg/mL) for the indicated times. Cell proliferation was determined by MTT assay. Data are shown as means ± S.E.M of three independent experiments. (b) Cells were treated with 100 μg/mL YSC-ZDC or 20 μg/mL 5-Fu for 48 h and then fixed with 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide. Ultrathin sections were examined using transmission electron microscopy. (c) Cells were treated with 100 μg/mL YSC-ZDC or 20 μg/mL 5-Fu for 48 h and incubated with Hoechst 33258 (5 μg/mL) for 10 min. Morphological changes of nuclear were visualized and analyzed using an inverted fluorescence microscope.

(a)

(b)

(c)