Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

Table 3

Simultaneous identification of antioxidant activity with online screening HPLC-ABTS assay.

Compounds	UV wavelength (nm)	Retention time (min)	Peak area (mAu)		Positive; S.D.	Negative; S.D.
			Positive (average)	Negative (average)
Gallic acid hydrate	210	5.623	72.116	51.624	0.054	0.405
(+)-Catechin hydrate	210	9.463	75.974	57.981	0.076	0.328
Caffeic acid	320	11.123	108.475	57.808	0.048	0.433
Rutin hydrate	210	15.860	51.185	13.241	0.393	0.023
Hyperoside	210	16.263	80.346	15.631	0.017	0.213
Quercetin	210	23.583	109.672	22.155	0.101	0.067
Kaempferol	254	28.303	56.806	30.651	0.143	0.071