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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 170858, 9 pages
http://dx.doi.org/10.1155/2015/170858
Research Article

Combined Effects of Muricid Extract and 5-Fluorouracil on Intestinal Toxicity in Rats

1Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia
2Basil Hetzel Institute, Queen Elizabeth Hospital, Woodville, SA 5011, Australia
3Gastroenterology Department, Women’s and Children’s Hospital, Adelaide, SA 5000, Australia
4School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Adelaide, SA 5371, Australia
5School of Biological Sciences, Flinders University, Adelaide, SA 5000, Australia
6Flinders Centre for Innovation in Cancer, Flinders University, Adelaide, SA 5000, Australia
7Marine Ecology Research Center, School of Environment, Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia

Received 19 February 2015; Revised 16 April 2015; Accepted 16 April 2015

Academic Editor: Jae Youl Cho

Copyright © 2015 Roger Yazbeck et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chemotherapy drugs, such as 5-fluorouracil (5FU), are the standard approach for cancer and are associated with several peripheral toxicities. We previously demonstrated that Muricidae marine molluscs exhibit chemopreventive properties. This study investigated the combined effect of muricid extract derived from Dicathais orbita, with 5FU, on intestinal toxicity in rats. Groups of rats were orally gavaged water, muricid extract, or sunflower oil, with or without 5FU (150 mg/kg). Metabolic data was collected daily and small intestinal brush border enzyme activity was measured by sucrose breath test (SBT). Blood was collected by cardiac puncture for whole blood analysis. Intestinal biopsies were taken for histopathology. Neutrophil activity was measured by myeloperoxidase activity. No additional toxicity effects were observed in rats receiving the combination of 5FU and muricid extract compared to 5FU alone, as indicated by SBT, histopathology, and myeloperoxidase activity. Intestinal integrity was protected from 5FU-induced damage in the sunflower oil vehicle group, compared to controls, as measured by SBT, villus height, and crypt depth. We concluded that combination of muricid extract and 5FU did not confer any additional intestinal toxicity, further supporting its potential as a chemopreventive food product. In this model system, sunflower oil partially protected against 5FU-induced intestinal toxicity.