Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 175497, 7 pages
http://dx.doi.org/10.1155/2015/175497
Research Article

In Vitro Antimicrobial and Antiproliferative Activity of Amphipterygium adstringens

1Institute of Biotechnology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, Avenida Universidad S/N, Ciudad Universitaria, CP 66455, San Nicolas de los Garza, NL, Mexico
2Escola Bahiana de Medicina e Saúde Pública (EBMSP), Avenida Silveira Martins 3386, 41150 100 Salvador, BA, Brazil
3Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), University of Campinas (UNICAMP), CP 6171, 13083-970 Campinas, SP, Brazil

Received 26 June 2015; Accepted 20 August 2015

Academic Editor: Rahmatullah Qureshi

Copyright © 2015 A. Rodriguez-Garcia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Amphipterygium adstringens is a plant widely used in Mexican traditional medicine for its known anti-inflammatory and antiulcer properties. In this work, we evaluated the in vitro antimicrobial and antiproliferative activities of the methanolic extract of A. adstringens against oral pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Candida albicans, and Candida dubliniensis, using microdilution (MIC) and agar diffusion methods (MBC), and the antiproliferative activity evaluating total growth inhibition (TGI) by staining the protein content with sulforhodamine B (SRB), using nine human cancer cell lines. Crude extract (CE) of A. adstringens showed some degree of activity against one or more of the strains with a MIC from 0.125 mg/mL to 63 mg/mL and MBC from 1.6 to 6.3 mg/mL and cytotoxic activity, particularly against NCI-ADR/RES, an ovarian cell line expressing multiple resistance drugs phenotype. The CE is a complex mixture of possible multitarget metabolites that could be responsible for both antimicrobial and antiproliferative activities, and further investigation is required to elucidate the identity of active compounds. Nevertheless the CE itself is useful in the development of new antimicrobial treatment based on natural products to prevent oral diseases and as alternative natural source for cancer treatment and prevention.