The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3K/Akt Pathway in Human Renal Proximal Tubular Epithelial Cells

Figure 6

The effect of apigenin on cisplatin-induced Akt phosphorylation in HK-2 cells. Cells were pretreated with different concentrations of apigenin for 1 h and then exposed to 40 μM of cisplatin for 8 h. The levels of Akt phosphorylation and expression were examined by Western blot analysis using anti-phospho-Akt (Ser473) and anti-Akt antibodies. The density of band was quantified by a densitometry using Bio-Rad Quantity One software. Value are means ± SD, . versus cells treated with cisplatin alone.